The use of anaerobic digestion or biodigestion has the potential to become a cost-effective waste management strategy that could be adopted by any industry where solid or semi-solid waste is generated. Biodigestion provides an opportunity for the upcycling of industrial waste into an alternative source of energy in the form of biogas, while reducing the environmental and economic burden derived from manufacturing processes, but it has not yet been fully applied. Biogas is a mixture of organic and inorganic gases composed primarily of methane (CH4), carbon dioxide (CO2), hydrogen sulfide (H2S) and trace gases (Chen et al., 2015)). We propose the use of biodigesters to carry out anaerobic digestion using organic substrates along with horse manure as the methanogenic inoculum. Complex biopolymers in substrates can be broken down to monomers and oligomers by the anaerobic methanogenic microorganisms found in the horse manure, resulting in the production of biogas. This[PBM(1] study aims to investigate total biogas production by volume and quality as a response to treatments with differing ratios of two combinations of substrates with varying nitrogen content in 1000-liter biodigesters charged with horse manure. Biogas quality was determined using gas chromatography by measuring the methane-to-carbon dioxide ratio resulting from each treatment in triplicate. The biogas quality results in the first set of quantitative analysis of CH4 and CO2 was presented as a percent ratio for treatment ratios 1:3, 1:1, and 3:1 (oak:soy) were 34:52, 36:41, and 35:35, respectively. The biogas quality for the second set of quantitative analysis for treatments 1:3, 1:1, and 3:1 (oak:soy) were 50:39, 43:33, and 48:36, respectively. Mean biogas production for the first set of quantitative analysis was determined to be approximately 160-820 liters[PBM(2] for treatments 1:3, 1:1, and 3:1 (oak:soy), respectively. Mean biogas production for the second set treatments was determined to be approximately 400-1900 liters for treatments 1:3, 1:1, and 3:1 (oak:soy).